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间歇静水压:软骨细胞的新陈代谢及发育,除了有必要的营养成分支持外,力学刺激也是必不可少的,而力学刺激又有许多方式,如剪切力、流水动力、持续压力、间歇压力等,这些力学刺激对软骨细胞的诱导分化结果不尽相同,而最接近生理状态下的力学刺激最有利于软骨细胞的发育与分化,间歇静水压就是模仿关节运动的方式,把细胞放在培养液里间断对细胞进行力学刺激,进而促进细胞分化和发育。
软骨组织工程:软骨组织损伤后很难再生,如何修复受损伤的软骨组织是目前国际关注的焦点,利用工程学原理,重新构建新的软骨组织,是修复软骨组织最有效的方法,但构建新的软骨组织非常复杂,需要能够分化成软骨细胞的干细胞,需要分化所必需的培养液、培养支架、力学环境等因素,还需要稳定的生长发育环境,因此从种子细胞到软骨细胞,最后形成软骨组织是一个复杂的生物工程。
背景:软骨组织修复是组织工程研究的重要领域,如何利用工程学技术有效将种子细胞定向分化成软骨细胞是组织工程的重点和难点。目前,单纯应用各种定向诱导培养试剂很难使其分化为成熟稳定的软骨细胞,正是在这一背景下,作者利用ATDC5软骨细胞的特点,除了应用有效的培养液处理外,还采用间歇净水压的压力刺激方法,对其定向诱导分化进行早期研究。
目的:了解间歇静水压对ATDC5软骨细胞早期软骨方向分化成熟的影响。
方法:将ATDC5软骨细胞株在单层条件下培养,3 d细胞贴壁良好,并形成复层,而后在密封条件下进行间歇静水压(施加强度10 MPa,加压频率1 Hz,4 h/d)培养,设立无间歇静水压且其他条件相同的培养细胞为对照组。在第4,7,11,14,17天,通过显微镜观察细胞形态变化,应用Real-time PCR检测Aggrecan,COL-2,SOX-9的mRNA表达水平。
结果与结论:经间歇静水压作用后,ATDC5细胞表现出较明显的斑块样改变和细胞浓聚现象;Aggrecan、COL-2 mRNA表达水平明显升高,SOX-9 mRNA虽然与对照组变化不大,但也出现了先抑后扬的特点。结果表明,间歇静水压影响ATDC5软骨细胞向软骨方向分化的基因表达,促进软骨特征基质的分泌,利于向软骨细胞分化成熟。
ORCID: 0000-0003-0911-8294(张强)
中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程 相似文献
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BackgroundPatients awaiting solid organ transplantation may develop anti-HLA antibodies after sensitization events such as transfusions, pregnancies, or previous transplantations. However, the effects of a particular sensitization event on HLA alloimmunization have not been well studied in parallel using cell-based assays and solid-phase assays. In this study, we evaluated and compare how different sensitization events affect the HLA antibody screening (HLA-Ab) and donor specific antibody (DSA) status in solid renal organ transplantation patients.MethodsHLA antibody (HLA-Ab) screening tests like complement-dependent cytotoxicity crossmatch (CDC-XM), flow cytometry crossmatch (FC-XM) and Luminex panel-reactive antibody (L-PRA) were performed in all 1066 patients (635 males and 431 females). If any of these tests turned out to be positive, a Luminex single antigen bead (L-SAB) assay was performed for DSA identification. Test positive rates and antibody strengths were analyzed according to the different sensitization events and gender.ResultsIn this study, HLA-Ab screening tests positive rates (L-PRA, FC-XM and CDC-XM) were significantly higher in patients with previous transplantation (73.91%, 100% and 56.52% p < 0.001), previous pregnancy (57.46%, 70.14% and 18.85% p < 0.001) or blood transfusion (27.33%, 35.55% and 7.33% p < 0.001) compared with patients without a sensitizing event (6.17%, 13.58% and 1.09). In this study, re-transplantation group showed significantly stronger antibody strength (DSA) than non sensitized group (class I and II MFI 11418.04, 17,837.78 vs class I and II MFI 2659, 3329; P < 0.001) and those with single sensitization events of transfusion (class I and II MFI 11418.04, 17,837.78 vs class I and II MFI 5790.26, 6004.16; P < 0.001) or pregnancy (class I & II MFI 11418, 17,837 vs class I and II MFI 8631.71, 7253.29; P < 0.001).ConclusionsPregnancy and blood transfused had high allo-immunization rate for class I HLA antigens. While re-transplantation patients had high allo-immunization rate for both the HLA classes (HLA- class I and HLA- class II). Re-transplantation group showed significantly stronger antibody strength, followed by pregnancy and then transfusion. 相似文献
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